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Advent of Proteomic Tools for Diagnostic Biomarker Analysis in Alzheimer’s Disease

Author(s):

Manisha Singh*, Surinder P. Singh, P.K. Dubey, Rachana R, Shalini Mani, Deepshikha Yadav, Mugdha Agarwal, Shriya Agarwal, Vinayak Agarwal and Harleen Kaur   Pages 1 - 13 ( 13 )

Abstract:


Locating remedies for Alzheimer’s disease (AD) is been majorly restricted by inefficiency to establish a definitive detection model for early stage diagnosis of pathological events. This current lapse in AD diagnosis also limits the therapeutic efficiency of the drugs, which might have been effective if given at the earlier stages of the disease. The indicated situation directs towards the burgeoned need for an effective biomarker technique that will help in early detection of AD and would be imminently useful to facilitate improved diagnosis and stimulate therapeutic trials. Till date the major biomarkers, specifically associated with AD detection may help in determining the early stage AD diagnosis and identifying alterations in cellular proteome, offering deeper insight into disease etiology. Currently existing multidisciplinary clinical diagnosis of AD is very tedious, expensive procedure and requires highly trained and skilled professionals who are rarely available outside the specialty clinics. Mutations in amyloid precursor protein (APP) or Presenilin 1 and 2 (PSEN1 and PSEN2) are some biomarkers acting as a critical check points for AD diagnosis. However, the presence of some associated biomarkers in cerebrospinal fluid (CSF) such as total-Tau (tTau), phosphorylated-Tau (pTau) 181 and Amyloid-β (Aβ) 1-42 using structural or functional imaging techniques are considered for confirmatory diagnosis of AD. Further, molecular diagnosis of AD incorporates various sophisticated techniques including immuno-sensing, machine learning, nano conjugation-based detections etc. In current review description, we had summarized the various diagnostic approaches taken and their relevance in mitigating out the long-standing vital urgency of targeted diagnostic tool for detection of AD.

Keywords:

Neurodegeneration, Dementia, Amyloid beta protein, Functional imaging, Immunosensing, Differential diagnosis.

Affiliation:

Department of Biotechnology, Jaypee Institute of Information Technology (JIIT) Noida, U.P, Bhartiya Nirdeshak Dravya Division, CSIR-National Physical Laboratory, New Delhi, Physico-Mechanical Metrology Division, CSIR-National Physical Laboratory, New Delhi, Department of Biotechnology, Jaypee Institute of Information Technology (JIIT) Noida, U.P, Department of Biotechnology, Jaypee Institute of Information Technology (JIIT) Noida, U.P, Bhartiya Nirdeshak Dravya Division, CSIR-National Physical Laboratory, New Delhi, Department of Biotechnology, Jaypee Institute of Information Technology (JIIT) Noida, U.P, Department of Biotechnology, Jaypee Institute of Information Technology (JIIT) Noida, U.P, Department of Biotechnology, Jaypee Institute of Information Technology (JIIT) Noida, U.P, Amity Institute of Biotechnology (AIB), Amity University, Noida, U.P



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