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Affinity Tags for Protein Purification

Author(s):

Vibhor Mishra*   Pages 1 - 10 ( 10 )

Abstract:


The affinity tags are unique proteins/peptides that are attached at the N- or C-terminus of the recombinant proteins. These tags help in protein purification. Additionally, some affinity tags also serve a dual purpose as solubility enhancers for challenging protein targets. By applying a combinatorial approach, carefully chosen affinity tags designed in tandem have proven to be very successful in the purification of single proteins or multi-protein complexes. In this minireview, I discuss the key features of the most commonly used affinity tags. I have classified the affinity tags into two significant categories, epitope tags, and protein/domain tags. The epitope tags are generally small peptides with high affinity towards a chromatography resin. The protein/domain tags often time double duty as solubility enhancers while aiding affinity purification. Finally, I discuss protease-based affinity tag removal strategies after purification.

Keywords:

proteins, affinity tag, purification, chromatography, protease

Affiliation:

Department of Biology, Indiana University, Bloomington, IN 47405



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