Corina Sarmiento and Julio A. Camarero* Pages 408 - 424 ( 17 )
Protein splicing domains, also called inteins, have become a powerful biotechnological tool for applications involving molecular biology and protein engineering. Early applications of inteins focused on self-cleaving affinity tags, generation of recombinant polypeptide α-thioesters for the production of semisynthetic proteins and backbone cyclized polypeptides. The discovery of naturallyoccurring split-inteins has allowed the development of novel approaches for the selective modification of proteins both in vitro and in vivo. This review gives a general introduction to protein splicing with a focus on their role in expanding the applications of intein-based technologies in protein engineering and chemical biology.
Inteins, split-inteins, protein modification, protein trans-splicing, protein splicing, post-translational modification, conditional protein splicing, backbone cyclized proteins.
Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA9033, Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA9033