Submit Manuscript  

Article Details


Biotechnological Applications of Protein Splicing

[ Vol. 20 , Issue. 5 ]

Author(s):

Corina Sarmiento and Julio A. Camarero*   Pages 408 - 424 ( 17 )

Abstract:


Protein splicing domains, also called inteins, have become a powerful biotechnological tool for applications involving molecular biology and protein engineering. Early applications of inteins focused on self-cleaving affinity tags, generation of recombinant polypeptide α-thioesters for the production of semisynthetic proteins and backbone cyclized polypeptides. The discovery of naturallyoccurring split-inteins has allowed the development of novel approaches for the selective modification of proteins both in vitro and in vivo. This review gives a general introduction to protein splicing with a focus on their role in expanding the applications of intein-based technologies in protein engineering and chemical biology.

Keywords:

Inteins, split-inteins, protein modification, protein trans-splicing, protein splicing, post-translational modification, conditional protein splicing, backbone cyclized proteins.

Affiliation:

Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA9033, Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA9033

Graphical Abstract:



Read Full-Text article