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Creatinine Deiminase: Characterization, Using in Enzymatic Creatinine Assay, and Production of the Enzyme

[ Vol. 20 , Issue. 5 ]

Author(s):

Andriy Zakalskiy*, Nataliya Stasyuk and Mykhailo Gonchar   Pages 465 - 470 ( 6 )

Abstract:


The goal of the review is description of the main characteristics of creatinine deiminase (CDI), an important bioanalytical tool for creatinine (Crn) assay. Crn is an essential metabolite for diagnostics of kidney disfunction and some other diseases, a biomarker to control the hemodialysis procedure, as well as an important analyte for sport medicine (estimation of general physiological status of athletes). We have described the important sources for CDI isolation, cloning of the corresponding gene, the construction of microbial recombinant strains, overproducing CDI, and characteristics of the enzyme from different microorganisms. There are reviewing also the new bioanalytical methods for quantitative determination of Crn, including enzymatic ones based on using CDI.

Keywords:

Creatinine deiminase, protein characterization, gene cloning, enzyme isolation, creatinine, creatinine enzymatic assay.

Affiliation:

Department of Analytical Biotechnology, Institute of Cell Biology, National Academy of Sciences of Ukraine, 14/16 Drahomanov Str., 79005, Lviv, Department of Analytical Biotechnology, Institute of Cell Biology, National Academy of Sciences of Ukraine, 14/16 Drahomanov Str., 79005, Lviv, Department of Analytical Biotechnology, Institute of Cell Biology, National Academy of Sciences of Ukraine, 14/16 Drahomanov Str., 79005, Lviv



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