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Mixed Mode Chromatography, Complex Development for Large Opportunities

[ Vol. 20 , Issue. 1 ]


Charlotte Cabanne* and Xavier Santarelli   Pages 22 - 27 ( 6 )


Mixed mode chromatography resins with salt tolerance, large design space and orthogonal selectivity requires a slightly more complex development than traditional resins. It is important to screen several ligands and several binding and elution conditions. This allows taking full advantage of these resins. High-Throughput Screening (HTS) for Process Development should be done with the help of Design of Experiment (DoE). It could be performed in filter plates or Robocolumns, and assisted by liquid handling automated workstation. Modeling of the results allows the choice of optimal parameters that can then be validated and scaled up. All this leads to a better knowledge and robustness of the purification step.


Mixed mode chromatography, high throughput process development, design of experiment, large opportunities, High-Throughput Screening (HTS), Design of Experiment (DoE).


Bordeaux INP, CBMN, UMR 5248, F-33600, Pessac, Bordeaux INP, CBMN, UMR 5248, F-33600, Pessac

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