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Review on the Application of Mixed-mode Chromatography for Separation of Structure Isoforms

[ Vol. 20 , Issue. 1 ]

Author(s):

Tsutomu Arakawa*   Pages 56 - 60 ( 5 )

Abstract:


Proteins often generate structure isoforms naturally or artificially due to, for example, different glycosylation, disulfide scrambling, partial structure rearrangement, oligomer formation or chemical modification. The isoform formations are normally accompanied by alterations in charged state or hydrophobicity. Thus, isoforms can be fractionated by reverse-phase, hydrophobic interaction or ion exchange chromatography. We have applied mixed-mode chromatography for fractionation of isoforms for several model proteins and observed that cation exchange Capto MMC and anion exchange Capto adhere columns are effective in separating conformational isoforms and self-associated oligomers.

Keywords:

Mixed-mode, isoform, glycoprotein, arginine, Capto MMC, Capto adhere.

Affiliation:

Alliance Protein Laboratories, A Division of KBI Biophama, 6042 Cornerstone Court West, San Diego, CA 92121

Graphical Abstract:



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